Fig. 1

Illustration of the Gsdma3 mutants and constructs used in this study. (a) Multiple amino acid sequence alignment of human GSDMA and mouse Gsdma1-3 using Vector NTI software. The yellow residues are identical; the blue residues are conserved; and the green residues are similar. The rectangular boxes indicate putative leucine zipper motifs. Nine conserved leucine-rich regions across the family members are indicated with horizontal bars with Roman numerals (based on [4]). The asterisk indicates the end of the N-Gsdma3 construct, which contains the leucine-rich region I-IV; the arrowhead marks the beginning of the C-Gsdma3 construct, which contains the leucine-rich region V-XI. The mutation sites in Gsdma3 are indicated with arrows with amino acid substitutions, and the type of mutation is shown in parentheses. The dotted underlining indicates the peptide sequence used to generate the rabbit polyclonal antibody against Gsdma3. (b) A schematic representation of the Gsdma3 and mutant Gsdma3 constructs. The N-terminal (A.A.1-227, hereafter referred to as N-Gsdma3) and C-terminal (A.A. 229-464, hereafter referred to as C-Gsdma3) portions are indicated. The gray box highlights the linker region between the putative N- and C-terminal domains. The different types of C-terminal tags are labeled