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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: N-terminal functional domain of Gasdermin A3 regulates mitochondrial homeostasis via mitochondrial targeting

Fig. 3

Coimmunoprecipitation analyses of the association between the N- and C-terminal domains of Gsdma3. (a, b) Immunoblot analysis of HEK293T cells transfected with the indicated plasmid constructs. Relative densitometry levels normalized to β-actin expressions are indicated. (c, d, e) Co-immunoprecipitation and immunoblot analysis of HEK293T cells transfected with the indicated plasmid constructs. Whole cell lysates (WCL) were split in half, immunoprecipitated and immunoblotted with the indicated antibodies. Asterisks indicate non-specific reactive bands. The molecular weight of the protein standards in kilodaltons (kDa) is indicated. Non-specific bands corresponding to the IgG heavy chain (IgGh) and light chain (IgGl) are indicated. Models of the putative domain interaction are listed in each panel

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