Construct name | Method | Primer sequences (5’ → 3’) |
---|---|---|
Gsdma3 | PCR cloning | forward, AGCGTCGACAGACAATGCCTGTGTTTGAGG; |
-pENTR2B | reverse, CCGGAATTCGAAGATAGAGCACAATAAGTAAGTGC | |
Gsdma3I359N -pENTR2B | mutagenesis | forward, GCTAACTGAAGAACAACTGAAGAATCTAGTAA AATCCTTGGAGA |
reverse, TCTCCAAGGATTTTACTAGATTCTTCAGTTGTTCTTCAGTTAGC | ||
Gsdma3M1Btlr | mutagenesis | forward, GCTAACTGAAGAACAACTGAAGAATCTAGTAA AATCCTTGGAGA; |
-pENTR2B | ||
reverse, TCTCCAAGGATTTTACTAGA TTCTTCAGTTGTTCTTCAGTTAGC | ||
Gsdma3M1Btlr | PCR cloning | forward, AGCGTCGACAGACAATGCCTGTGTTTGAGG; |
-pENTR2B | ||
reverse, GCGGA ATTCCCCTCCAAGGATTTTACTAG | ||
N-Gsdma3 | PCR cloning | forward, AGCGAATTCAGACAATGCCTGTGTTTGAGG; |
-pCDNA3.1-myc-His(-)A | reverse, AGCGAATTCCGCTGTCATTGCAAATGTACGG | |
C-Gsdma3 | PCR cloning | forward, AGCGAATTCATATGCAAACCTTCCCTAAGATCAGG; |
-pCDNA3.1-myc-His(-)A | reverse, CCGGAATTCCAAGATAGAGCACATAAGTAAGTGC | |
C-Gsdma3Rim3 | PCR cloning | forward, AGCGAATTCATATGCAAACCTTCCCTAAGATCAGG; |
-pCDNA3.1-myc-His(-)A | reverse, CCGGAATTCCAAGATAGAGCACATAAGTAAGTGC | |
Gsdma3 | PCR cloning | forward, TCCGCGGCCGCACCATGCCTGTGTTTGAGGAT; |
-pIRES-hrGFP-1a | ||
reverse, CCGGAATTCCAGATAGAGCACAATAAGTAA | ||
Gsdma3Rim3 | PCR cloning | forward, TCCGCGGCCGCACCATGCCTGTGTTTGAGGAT; |
-pIRES-hrGFP-1a | ||
reverse, CCGGAATTCCAGATAGAGCACAATAAGTAA | ||
N-Gsdma3 | PCR cloning | forward, TCCGCGGCCGCACCATGCCTGTGTTTGAGGAT; |
-pIRES-hrGFP-1a | ||
reverse, CCGGAATTCCGCTGTCATTGCAAATGTACGG | ||
C-Gsdma3 | PCR cloning | forward, TCCGCGGCCGCACGATGCAAACCTTCCCTAAG; |
-pIRES-hrGFP-1a | ||
reverse, CCGGAATTCCAGATAGAGCACAATAAGTAA | ||
C-Gsdma3 (no tag) | PCR cloning | forward, AGCGAATTCATATGCAAACCTTCCCTAAGATCAGG; |
-pCDNA3.1-myc-His(-)A | reverse, CCGGAATTCAAAGATAGAGCACATAAGTAAGTGC | |
C-Gsdma3Rim3 (notag) | PCR cloning | forward, AGCGAATTCATATGCAAACCTTCCCTAAGATCAGG; |
-pCDNA3.1-myc-His(-)A | reverse, CCGGAATTCAAAGATAGAGCACATAAGTAAGTGC |