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Table 2 List of the primary antibodies and dilutions used for western blotting/ immunoprecipitation (WB/IP) and immunostaining (ICC)

From: N-terminal functional domain of Gasdermin A3 regulates mitochondrial homeostasis via mitochondrial targeting

Antigen

Antibody

Species

Company

WB/IP

ICC

V5

monoclonal

mouse sv5-pk

Santa Cruz

1:500/IP(2 μg)

1:300

V5

polyclonal

rabbit

Genetex

1:5000

1:200

myc

monoclonal

mouse 9E10

Santa Cruz

1:1000/IP(2 μg)

1:200

flag

monoclonal

mouse M2

Agilent Tech

1:2000/IP(2 μg)

 

Cox4

polyclonal

rabbit

Genetex

1:1000

 

Tom20

polyclonal

rabbit

Santa Cruz

 

1:500

Gsdma3

polyclonal

rabbit

Home made

1:500

1:200

β-actin

polyclonal

rabbit

Genetex

1:5000

 

GAPDH

Polyclonal

rabbit

Genetex

1:5000

 

Hsp90α/β

polyclonal

rabbit

Santa Cruz

IP (2 ug)

 

Hsp90β

polyclonal

rabbit

Genetex

1:2000

 

Hsp70 1A

polyclonal

polyclonal rabbit

Genetex

1:2000

 

STIP1/Hop

polyclonal

rabbit

Genetex

1:2000

 

TRAP1

monoclonal

mouse

Genetex

IP (2 ug)

 

TRAP1

polyclonal

rabbit

Genetex

1:4000

 

AKT1

polyclonal

rabbit

Millipore

1:2500

 

Tom70

monoclonal

mouse

Santa Cruz

1:2000/IP(2 ug)

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