Fig. 5
From: In vivo imaging of endogenous enzyme activities using luminescent 1,2-dioxetane compounds
CIEEL energy transfer imaging of endogenous phosphatase activity in cells. Human MM1S myeloma cells were stained with DiR prior to CIEEL imaging with CSPD, a chemiluminescent 1,2-dioxetane substrate for alkaline phosphatase detection. Unstained cells were used as negative control. Luminescence emission levels were determined by sequential scanning from 500 to 840 nm. Quantitative representation of the imaging data showed DiR-mediated energy transfer signals in the 720–840 nm NIR region, a finding consistent with the DiR’s fluorescent emission profile