Fig. 5
Effect of melatonin pre-treatment on sperm viability and motility in mice exposed to whole-body 60Co γ-irradiation. Animals were sacrificed through cervical dislocation and sperm was collected from cauda-epididymis after 2h, 4h and 8h post-irradiation. Sperm cells were stained with Eosin-Y and viable sperm cells were analyzed using haemocytometer. Immediately, after sacrificed, sperm cells motility was analyzed using haemocytometer. Results are represented as viability index and percentage motility index for viable and motile sperm cells, respectively. *p < 0.001, **p < 0.05