Fig. 3

Intraperitoneal LPS infusion induces a COX-2-dependent and redox-sensitive increase in tissue superoxide levels in the hippocampus. Tissue level of superoxide anion, measured by electron paramagnetic resonance (EPR) spectroscopy, in the hippocampus on day 7 after intraperitoneal infusion via an osmotic minipump of saline or LPS (2.5 mg/kg/day) for 7 days alone with additional intracerebroventricular infusion NS398 (5 μg/μl/h, dissolved in 1% DMSO), tempol (2.5 μg/μl/h, dissolved in saline) or the corresponding vehicle. The concentration of superoxide in arbitrary unit was derived from computer simulation of the EPR spectra by normalized line shapes. Values are mean ± SEM, n = 8–10 rats per group. *P <0.05 vs. saline-treated group; ^#P <0.05 vs. LPS-treated group in the post hoc Scheffé multiple-range test