Fig. 7
From: Role of Raf-kinase inhibitor protein in colorectal cancer and its regulation by hydroxycamptothecine
Effect of RKIP on sensitivity of CRC cells to hydroxycamptothecine (HCPT); a RKIP expression in each cell group. SW1116 con: SW1116 cells transferred with empty pIRES2-EGFP vector; HCPT con: SW1116/HCPT cells transferred with empty pIRES2-EGFP vector; SW1116 271: SW1116 cells transferred with shRNA vector targeted RKIP(PGPU6/GFP/Neo-PEBP1-271); HCPT PEBP-1: SW1116/HCPT cells transferred with over-expression pIRES2-EGFP/PEBP-1 vector; b 5 × 103 cells incubated in 96-well culture plate for 24 h followed by treatment with different concentrations of HCPT for 48 h. Cell survival rate determined by MTT assay. IC50 values of HCPT analyzed by SPSS software. Abbreviations: RKIP, Raf kinase inhibitor protein, HCPT, hydroxycamptothecine; MTT: (3-(4,5-dimethylthiazol-2-yl)-2-5 diphenyl tetrazolium bromide) assay