Fig. 4

Assessment of apoptosis as cell death mechanism induced by CDKs inhibitors. a Analysis of Annexin V-positive cells on Imatinib-sensitive K562 (a), KCL22 (c), and BaF3 Bcr-Abl WT (e) and Imatinib-resistant K562-R (b), KCL22-R (d), and BaF3 Bcr-Abl T315I (f) CML cell lines treating with increasing concentrations of drugs for 48 h. Negative and positive controls of cell viability were obtained with etoposide-treated or untreated cells, respectively. b Analysis of K562 and KCL22 Annexin V-positive cells treated for 24, 48 or 72 h with 10 μM drugs. c DNA fragmentation analysis of KCL22 cells treated by CDK inhibitors. NT not treated, I Imatinib, R Roscovitine, R8 R-CR8, S8 S-CR8, MR4 MR4, D DMSO, MW molecular weight