Fig. 8

Effects of CDKs inhibitors on ROS generation in CML cell lines. a ROS were detected by DHE probe on KCL22 cells treated with increasing concentrations of Roscovitine or R-CR8: 1 μM (light grey), 2 μM (dark grey), and 10 μM (black). B, KCL22 were treated by 10 μM Roscovitine or R-CR8 for 4 h (white bars) or 24 h (black bars) before measurement of ROS. C, K562 (white bars) and KCL22 (black bars) cells were treated by 2 μM R-CR8 after pretreatment with 50 μM caspase inhibitors. All results are expressed as percent of DHE-positive cells