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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Neurotensin-polyplex-mediated brain-derived neurotrophic factor gene delivery into nigral dopamine neurons prevents nigrostriatal degeneration in a rat model of early Parkinson’s disease

Fig. 2

Transgenic expression in substantia nigra (SN) of hemiparkinsonian rats at week 2 after transfection using NTS-polyplex. a Representative photograph of RT-PCR amplicons showing BDNF-flag and GFP expression. Actin was used as a control. Lane 1, plasmid phDAT-BDNF-flag or phDAT-EGFP (positive control); lane 2, PCR reagents without cDNA (internal control); lane 3, SN (substantia nigra) with lesion and transfected with phDAT-BDNF-flag or phDAT-EGFP; lane 4, contralateral SN without lesion and transfection; lane 5, striatum ipsilateral to the side with lesion and nigral transfection; lane 6, contralateral striatum. Representative confocal micrographs showing TH-immunoreactivity (IR) and flag-IR (b) or green fluorescent protein (GFP)-IR (c) in the substantia nigra (SN) of hemiparkinsonian rats. The control side are micrographs of the intact SN contralateral to the side with the lesion and transfection (Experimental side). d Graph showing the percentage of flag or GFP expression with respect to the total TH+ cells. n = 3 rats per each experimental condition. One-way ANOVA and Bonferroni post hoc test. *** P < 0.0001

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