Fig. 4

Significant recovery of TH+ fibers in the striatum of hemiparkinsonian rats at week 2 after transfection with the phDAT-BDNF-flag using NTS-polyplex. Representative micrographs of TH-immunohistochemistry of the striatum of hemiparkinsonian rats at three weeks after a 6-OHDA striatal lesion, and two weeks after applying one of the following treatments in the ipsilateral substantia nigra: without transfection (NT), Dulbecco’s Modified Eagle Medium injection (DMEM), phDAT-EGFP (GFP), or phDAT-BDNF-flag (BDNF) transfection(a-e. Illustrations of image processing using ImageJ software for quantification of TH-immunoreactivity density (f-j). The bar graph shows TH-immunoreactivity density (k) in the experimental conditions as mentioned above. Values are expressed as the mean ± SE; n = 3 animals per group. ** P < 0.001 when compared with the intact side. † P < 0.001 when compared with the negative control groups. One-way ANOVA with Bonferroni’s post hoc test