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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Osthole ameliorates hepatic fibrosis and inhibits hepatic stellate cell activation

Fig. 3

Osthole suppressed inflammation and oxidation in TAA-injected rat liver. a Nuclear fractions of rat liver from different treatment groups were analyzed by Western blotting analysis. For expression of p65, JunD and Nrf-2, PCNA expression served as loading control in nuclear protein. b GSH/GSSG ratio in the livers was measured by Glutathione kit. c Levels of 4-HNE in the livers were measured using 4-HNE ELISA kit. d Levels of MDA in the livers were measured using MDA ELISA kit e qRT-PCR showed the expressions of interleukin-1β, tnf-α and inos transcripts in rats. f ELISA of LIX, CXCL1, CCL20 and CCL5 in plasma. Data are shown as mean ± SD of 6 rats in each group. *p < 0.05; **p < 0.01, compared with other groups

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