Fig. 2

5’-AMP-induced whole body cooling treatment increases stability and glycosylation of ΔF508-CFTR in intestinal epithelium - Western analysis of intestinal duodenal epithelium. a CF mice given four treatment cycles every 72 h, with each cooling cycle lasting 7–10 h; b CF mice that had undergone one cooling treatment lasting 12–17 h. All mice were sacrificed 24 h after the last treatment. Each gel lane represents a tissue sample from an individual mouse. The left and right photograph reflects short and longer film exposures of the respective blot. Intestinal epithelia from three wild type and three untreated CF mice were used as positive and negative controls for both Western analyses. A nonspecific 72 kDa band that immune reacted with the antibody preparation was used as an internal control (IC) for loading samples