Fig. 1

JEV-based replicons and JEV-CprME packaging cells. a Schematic diagrams of JEV-based replicons. JEV infectious cDNA driven by CMV promoter encodes three structural (gray box) and seven nonstructural (open boxes) proteins. JRP was derived from JEV infectious cDNA by deletion of nucleotides from 198–2387, where most of structural proteins were deleted. JRPSP70 included a SP70 epitope (red box) that was fused at C-terminal of NS1. b The packaging cells, named JCprME, were created by using pseudo-lentiviral particles containing structural genes CprME to infect Vero cells and were selected by puromycin. The E-expression of JCprMC cells was determined by Western blot (left panel) and IFA (right panel) using MAb against JEV E protein, and Vero cells served as a negative control. Samples from rRP-9-infected lysates serve as a positive control for protein position of E. The arrow denotes the position of E proteins. Scale bar is 50 μm in length