Skip to main content
Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Japanese encephalitis virus replicon-based vaccine expressing enterovirus-71 epitope confers dual protection from lethal challenges

Fig. 3

Multimeric NS1 fusion proteins derived from SRIP-SP70 infection. JCprME cells were repeatedly infected by SRIP-SP70 as indicated, and cell lysates were prepared for detection of the intracellular NS fusion proteins (a) and supernatants were collected for detection of the extracellular NS fusion proteins (b). Analyzed by Western blotting using NS1-specific or SP70-specific antibodies, the protein samples from cell lyastes or supernatants were left unboiled to detect multimeric NS1 fusion proteins (the left parts of panels a and b), or being boiled to examine monomers of NS1 proteins (the right parts of panels a and b). Samples from virus infection by rRP-9 or rRP9-SP70 serve as a positive control. Samples from JCprME were mock-infected controls. The arrow denotes the dimer or monomer position of NS1 or NS1-SP70

Back to article page