Fig. 2

TAKI-induced cytotoxicity is dependent on RIP1 activity. (a) BMDM were treated with TAKI (100 nM) and/or LPS (100 ng/ml) for indicated time periods. Cell lysates were collected for immunobloting. (b, c) BMDM were pre-treated with necrostatin-1 (Nec-1, 10 μM) and/or zVAD (20 μM) for 30 min followed by adding TAKI (100 nM) and/or LPS (100 ng/ml) for 6 h. LDH activity in the collected culture medium (b) and amount of Annexin V/PI positive cells (c) were determined. *p <0.05, indicating the significant effect of TAKI alone. #p <0.05, indicating the significant effects of LPS and zVAD on the response of TAKI. **p <0.05, indicating the significant inhibition of TAKI-induced cell death by Nec-1