Fig. 4

Western Blot Analysis for Cell Cyclins. Protein expressions of cyclins E and D1 in lungs exposed to different doses of cigarette smoke for 6 weeks. Representative Western Blot analyses are shown that used anti-cyclin E, and anti-cyclin D1 antibodies. The size of the detected cyclin E and D1 were 50 kDa and 36 kDa, respectively. The intensity of the protein signal was determined by densitometry analysis (three samples in each group). The relative protein values in the three treatment groups were calculated as a mean value ± standard deviation (SD) of the data relative (ratio) to control with the control set at 1.0. Different letters represent significant difference among groups (P < 0.05)