Fig. 3

Epididymal fat deposition, miR-130b and its target gene PPAR-γ expression. a: Flow chart depicting the experimental design. The obese C57BL/6 mice were injected via tail vein with miR-SC-MV or miR-130b-MV every other day for 10 days. miR-130b-MV was harvested by transfecting HeLa-229 cells with miR-130b plasmid. Ten days later, the mice were weighed and sampled. b: Gene expression of miR-130b in miR-130b-MV. c: Body sizes. d-e: Epididymal fat sizes. f-g: Epididymal fat weight and the ratio between epididymal fat weight and body weight. h: Expression of miR-130b gene in the epididymal fat. i: mRNA expression of the miR-130b target genes. j: mRNA expression of the miR-130b target gene PPAR-γ. k: Protein expression of miR-130b target gene PPAR-γ. HF, high fat; HF-SC-MV, high-fat diet-induced obese mice injected with miR-SC-MV; HF-130b-MV, high-fat diet-induced obese mice injected with miR-130b-MV; The values shown represent the means ± SEM, n = 12; *P < 0.05 vs. HF-SC-MV group; **P < 0.01 vs. HF-SC-MV group