Table 1 Primers used for plasmids construction and mRNA quantification
Name | Sequence |
|---|---|
Plasmids construction | |
ssc-miR-130b (MI0013136) | F: GATCCGCCTGCCTGACACTCTTTCCCTGTTGCACTACTG |
TGGGCCACTGGGAAGCAGTGCAATGATGAAAGGGCATCA | |
GTCAGGCTTTTTTGGAAA | |
R: AGCTTTTCCAAAAAAGCCTGACTGATGCCCTTTCATCAT | |
TGCACTGCTTCCCAGTGGCCCACAGTAGTGCAACAGGGA | |
AAGAGTGTCAGGCAGGCG | |
ssc-miR-SC | F: GATCCGACTTACAGCCAGTTCCTAGTATAGTGAAGCAG |
CAGATGGTATACTAGGAACTGGCTGTAAGCTTTTTTTGGA | |
AA | |
R: AGCTTTTCCAAAAAAAGCTTACAGCCAGTTCCTAGTA | |
TACCATCTGCTGCTTCACTATACTAGGAACTGGCTGTAAGTCG | |
mRNA expression | |
PPAR-γ (NM_138711) | F: GCCCTTCACCACTGTTGATT |
R: GAGTTGGAAGGCTCTTCGTG | |
GR (AY779185) | F: CCAAACTCTGCCTTGTGTGTTC |
R: TGTGCTGTCCTTCCACTGCT | |
TNF-α (NM_013693.3) | F: CTATGGCCCAGACCCTC |
R: GCAGCCTTGTCCCTTGA | |
UCP-3 (NM_009464.3) | F: ACGATGGATGCCTACAGGAC |
R: TCCGAAGGCAGAGACAAAGT | |
LDLR (NM_001252658.1) | F: TCAGTCCCAGGCAGCGTAT |
R: TGATCTTGGCGGGTGTT | |
STAT3 (NM_011486.4) | F: ATTGTGATGCCTCCTTGA |
R: ATTGGCGGCTTAGTGAA | |
FAS (EF589048) | F: GTCCTGCTGAAGCCTAACTC |
R: TCCTTGGAACCGTCTGTG | |
SCD-1 (NM_213781) | F: CCCAGCCGTCAAAGAGAA |
R: CGATGGCGTAACGAAGAAA | |
11β-HSD1 (AF414124) | F: CCATGCTGAAGCAGAGCAAC |
R: AAGAACCCGTCCAGAGCAAA | |
HSL (AY686758) | F: ACCCTCGGCTGTCAACTTCTT |
R: TCCTCCTTGGTGCTAATCTCGT | |
ATGL (EF583921) | F: ACCTGTCCAACCTGCTGC |
R: GCCTGTCTGCTCCTTTATCCA | |
MGL (NM_001166249.1) | R: CATTGCTCGCTCCACTCTT |
F: ATGGTCCTGATTTCACCTCTG | |
Leptin (NM_008493.3) | F: CCCTCATCAAGACGATTGTCA |
R: GGTTCTCCAGGTCATTCGATA | |
LeptinR (NM_001122899.1) | F: CCCTCATCAAGACGATTGTCA |
R: GGTTCTCCAGGTCATTCGATA | |
ACC (NM_133360.2) | F: AGCAGTTACACCACATACAT |
R: TACCTCAATCTCAGCATAGC | |
SREBP-1 (NM_011480.3) | F: GCTTCTCTTCTGCTTCTCT |
R: GCTGTAGGATGGTGAGTG | |
PPIA (NM_214353.1) | F: TCCTCCTTGGTGCTAATCTCGT |
R: TGATCTTCTTGCTGGTCTT |