Table 2 primers used for miRNA detection
Name | Primer sequence (5’ to 3’) | miRbase Acc No. |
|---|---|---|
miR-130b | CAGUGCAAUGAUGAAAGGGCAU | MIMAT0013922 |
miR-130a | CAGUGCAAUGUUAAAAGGGCAU | MIMAT0007758 |
miR-27a | UUCACAGUGGCUAAGUUCCGC | MIMAT0002148 |
miR-27b | UUCACAGUGGCUAAGUUCUGC | MIMAT0013890 |
miR-103 | AGCAGCAUUGUACAGGGCUAUGA | MIMAT0002154 |
miR-143-3P | UGAGAUGAAGCACUGUAGCUC | MIMAT0013879 |
miR-143-5P | GGUGCAGUGCUGCAUCUCUGG | MIMAT0017374 |
miR-378a | ACUGGACUUGGAGUCAGAAGGC | MIMAT0013868 |
miR-378b-3p | ACUGGACUUGGAGUCAGAAGU | MIMAT0037082 |
miR-455 | UAUGUGCCUUUGGACUACAUCG | MIMAT0022957 |
miR-320 | AAAAGCUGGGUUGAGAGGGCGAA | MIMAT0013878 |
miR-106a | AAAAGUGCUUACAGUGCAGGUAGC | MIMAT0002118 |
poly(T) adapter | TAGAGTGAGTGTAGCGAGCACAGAA | N/A |
TTAATACGACTCACTATAGGTTTTTT | ||
TTTTTTTTTTVN | ||
Universal primer | TAGAGTGAGTGTAGCGAGCA | N/A |
Exogenous reference | GTGACCCACGATGTGTATTCGC |