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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Involvement of gelsolin in TGF-beta 1 induced epithelial to mesenchymal transition in breast cancer cells

Fig. 3

Effects of GSN op on changes of cell morphology, cell surface elasticity, cytoskeletal protein expression, cell detachment, and migration in MDA-MB231 breast cancer cells. a GSN op altered cell morphology and (b) increased cell surface elasticity in MDA-MB231 cells. Four different stable GSN transfected MDA-MB231 cell clones were used. The calibration bar is 200 μm. Atomic force microscopy was applied to obtain with quantitative analysis of the cell-surface adhesion force in control and GSN op MDA-MB231 cells. c Effect of GSN op on cell detachment for MCF-7 cells. d Effect of GSN op on the protein levels shown by immunoblotting (top) with quantitative analyses (bottom) for caldesmon (CaD), tropomyosin 1 (Tm 1), GSN and profilin (Pro) in MDA-MB231 cells. β-actin used as an internal control. e Effect of siTm 1 on cell detachment for MDA-MB231cells with or without GSN op. f Effect of GSN on the cell migration in MDA-MB 231 cells. In b, c, d, e, f, the values are the mean ± SEM (n = 40 in b, n = 6 in c, d, e, f), with *, # indicating a significant difference compared to the cells in control and GSNop-siTm, respectively

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