Figs. 3

Western blot analysis of ERK 1/2 phosphorylation in HT-22 hippocampal neurons at different times (15, 30, 60 and 180 min) after incubation with GK-6 (10-6M), GK-2 (10-8M) or NGF (10-9M). Results were calculated as the ratio of arbitrary densitometric units of phosphorylated target protein to total non-phosphorylated target protein. Total nonphosphorylated target protein arbitrary densitometric units were analyzed separately against β-actin. Data are presented as means±SD of five independent experiments. Differences from the control were significant at *-р < 0.05 (Mann–Whitney U test)