Fig. 2

Crosslinking FcγRIIB inhibits cell viability of purified primary PCs and subsequent PC differentiation in vitro. a Purified PCs (10⁶/ml) were cultured for 24 h in the absence (circle) or presence of various doses (2–10 μg/ml) of isotype (squares) or AT10 (filled squares) mAbs. The number and percentage of dead cells were determined by staining with 7-AAD and side scattering using flow cytometry. b Primarily isolated CD19⁺ B cells (10⁶/ml) were cultured for 24 h as in (a) in 96-well ELISPOT plates as described in Methods. c Purified B cells from 6 donors were cultured as in (b) with the addition of CpG (5 μg/ml) alone, CpG + IL-2 + IL-10 or CpG + SAC + PWM for three or five days without antibody or in the presence of an isotype matched control mAb or AT10 mAb (5 μg/ml). The asterisks indicate that the differences between the groups compared are statistically significant (P < 0.05)