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Fig. 8 | Journal of Biomedical Science

Fig. 8

From: FcγRIIB mediates antigen-independent inhibition on human B lymphocytes through Btk and p38 MAPK

Fig. 8

FcγRIIB inhibits the proliferation of CD27 naïve B cells but not CD27 + memory B cells. a CD27+ and CD27 B cells (106/ml) were cultured for two days before the addition of 3[H] methyl thymidine for another 16 h. Antibodies (5 μg/ml) and stimuli were added to cells in the beginning of culture as in Fig. 6. A representative histogram of more than 3 donors was shown. The asterisks indicate that the differences between the groups compared are statistically significant (P < 0.05). Similar results were obtained when cells were cultured for 1–2 more days before adding 3[H] methyl thymidine. b CD27+ and CD27 B cells (105/ml) from six donors were culture for 48 h in the presence of 2 μg/ml of F (ab’)2 anti-Ig (IgM + IgG + IgA, Jackson Immunoresesearch) with (open circle) or without (closed circle) 10 μg/ml of ICs. Cells with decreased green fluorescence, indicative of cell division, were counted using flow cytometry and the absolute number (× 102) was plotted as the Y-axis. The horizontal bar in each group represents mean of subjects and the asterisks indicate that the differences between the groups compared are statistically significant (P < 0.05)

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