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Fig. 9 | Journal of Biomedical Science

Fig. 9

From: Soluble epoxide hydrolase inhibitor enhances synaptic neurotransmission and plasticity in mouse prefrontal cortex

Fig. 9

The sEH inhibitor AUDA induced the phosphorylation of ERK in PFC slices. a The PFC slices were incubated with AUDA (10 μM) for 10 min and then washed to remove AUDA. One hour later, homogenate from the PFC was prepared and blotted with antibodies directly against the active form of ERKs. AUDA-induced increase of ERKs phosphorylation in the PFC slices is shown. AUDA treatment increased the phosphorylated levels of ERK42 and ERK44 relative to the vehicle controls (ACSF). No change was observed when the cellular extract was blotted with an antibody that recognizes total ERKs, suggesting that the observed pERKs increments were not due to an increase in the total amount of ERKs. b Here shows the phosphorylated levels of ERK42 and ERK44 among the vehicle control, vehicle/HFS and AUDA/HFS groups. c The homogenate from the PFC following AUDA treatment was prepared and blotted with antibodies directly against the COX-2. GAPDH was the internal control *p < 0.05, **p < 0.01 vs. vehicle control; #p <0.05 vs. vehicle HFS group

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