Fig. 4

DNA methylation of CpG islands and CpG dinucleotide regions in myocardial cells with or without treatment of 5-azacytidine. Myocardial cells were isolated from 14-day-old mouse hearts. One group was treated with 5-azacytidine and another group was treated with medium without 5-azacytidine as the control. a MSP results of CpG island methylation in ssTnI promoter. The CpG islands are methylated both in blank and 5 μM 5-azacytidine treated groups. b MSP results of CpG dinucleotide region methylation in ssTnI promoter region -100 bp away from ssTnI TSS. The MSP data show that the methylation level on CpG sites in -100 bp of ssTnI TSS is reduced in cells treated with 5-azacytidine. M, methylated; U, unmethylated. c Summary of the Bisulfite sequence data of methylation on CpG island in ssTnI promoter, indicating that there is no methylation change either in cells treated with 5-azacytidine or in control cells. d Summary of the Bisulfite sequence data of methylation on CpG dinucleotide region in ssTnI promoter area -100 bp away from the ssTnI TSS. The results show a significant decrease of DNA methylation on these regions in the cells treated with 5-azacytidine. *p < 0.05