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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Unconserved C terminal of human cytomegalovirus tegument protein pUL76 elicits nuclear aggresome formation and induces DNA damage in transfected cells

Fig. 2

Assessment of the aggresome determinant region within UL76 (top panel) and transfection efficiency of each group (bottom panel). HEK293T cells were transiently expressed control EGFP and the fluorescent fusion proteins EGFP-UL76, EGFP-UL76N(1–190), and EGFP-UL76C(187–325). Fluorescent signals were accumulated into globular aggresome in cells transfected with pEGFP-UL76 (b) or pEGFP-UL76C (d). But the fluorescence intensities were diffusely distributed throughout most of the cells in pEGFP-N1 (a) and pEGFP-UL76N (c) groups. This indicates the unconserved C terminal of UL76 was the determinant region of its ability to elicit aggresome formation. The degradation of EGFP fusion proteins hampered our aim to measure the real expression level of UL76 and its deletion mutants by western blot. The ratio of EGFP positive cells in each transfection group may reflect the real expression level. Results of flow cytometry show that the EGFP+ ratio of each transfection group are: 66.2 % for pEGFP-N1, 36.4 % for pEGFP-UL76, 42.9 % for pEGFP-UL76N and 35.4 % for pEGFP-UL76C

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