Fig. 4

Effects of caffeine on hepatic lipid accumulation in zebrafish larvae overfed for 20 days. Frozen section hematoxylin and eosin staining (Frozen H&E) of liver sections in 30 mg/d (a), 180 mg/d (b), 1 % caffeine (c), 2.5 % caffeine (d), 5 % caffeine (e), and 8 % caffeine (f) feeding zebrafish larvae. Black arrow indicate lipid droplet. Frozen Oil red O staining (Frozen ORO) of liver sections in 30 mg/d (g), 180 mg/d (h), 1 % caffeine (i), 2.5 % caffeine (j), 5 % caffeine (k), and 8 % caffeine (l) feeding zebrafish larvae. Blue arrow indicate lipid droplet