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Table 1 Primer sequences used for quantitative real-time PCR (qPCR)

From: Caffeine reduces hepatic lipid accumulation through regulation of lipogenesis and ER stress in zebrafish larvae

Gene Accession Forward primer Reverse primer
PPAR-γ DQ839547 5′-GGTTTCATTACGGCGTTCAC-3′ 5′-TGGTTCACGTCACTGGAGAA-3′
SREBP1 NM_001105129 5′-CATCCACATGGCTCTGAGTG-3′ 5′-CTCATCCACAAAGAAGCGGT-3′
PPAR-α NM_001102567 5′-CGTCGTCAGGTGTTTACGGT-3′ 5′-AGGCACTTCTGGAATCGACA-3′
ACC1 XM_009298360 5-′GCATAGGGCAGGTTTTACCA-3′ 5′-GCCATCATACGAGAGCAACA-3′
FASN XM_009306807 5-′GAGAAAGCTTGCCAAACAGG-3′ 5′-GAGGGTCTTGCAGGAGACAG-3′
CD36 NM_001002363 5′-AGGCCACTGTGAACCTGAAG-3′ 5′-AAGTTGGGGTTCATTCCGAC-3′
UCP2 NM_131176 5′-GAGCTTTGCTTCTGTACGCA-3′ 5′-ACGAAACCCCTCTTCCTTTG-3′
CPT I NM_001044854 5′-ACTCTCGATGGACCCTGTGA-3′ 5′-CTGGATGAAGGCATCTGGAC-3′
ACO NM_213147 5′-AAGGACATCGAGCGAATGAT-3′ 5′-CTATGAAAGAGTGGAGGCCG-3′
ATF6 NM_001110519 5′-CTGTGGTGAAACCTCCACCT-3′ 5′-CATGGTGACCACAGGAGATG-3′
IRE1 XM_009306258 5′-TGACGTGGTGGAAGTTGGTA-3′ 5′-ACGGATCACATTGGGATGTT-3′
PERK XM_005156585 5′-TGGGCTCTGAAGAGTTCGAT-3′ 5′-TGTGAGCCTTCTCCGTCTTT-3′
BIP NM_213058 5′-AAGAGGCCGAAGAGAAGGAC -3′ 5′-AGCAGCAGAGCCTCGAAATA -3′
CHOP XM_005166171 5′-AAGGAAAGTGCAGGAGCTGA -3′ 5′-TCACGCTCTCCACAAGAAGA- 3′
Beclin-1 NM_200872 5′-AGAGCATTGAGACAAAGCGTGAA -3′ 5′-TCTGCCAAGGCGGAAGTTATT-3′
ATG12 BC154046 5′-TTCATCTCACGCTTCCTCAA-3′ 5′-CGTCACTTCCGAAACACTCA-3′
ATG3 NM_200022 5-′GGCTGTTTGGATATGATGAG-3′ 5′-AGCAGGTGGAGGGAGATTAG- 3′
IL-1β AY340959 5′-TGGCGAACGTCATCCAAG-3′ 5′-GGAGCACTGGGCGACGCATA-3′
TNF-α AY427649 5′-GCTTATGAGCCATGCAGTGA-3′ 5′-TGCCCAGTCTGTCTCCTTCT-3′
NF-κB BC162402 5′-GAAGCATTCAGGCTCGGTGA-3′ 5′-CAGGTCTGTCGGTCCCTTTC-3′
EFL1-alpha AY422992 5′-TACTTCTCAGGCTGACTGTG-3 5′-ATCTTCTTGATGTATGCGCT-3′