Skip to main content

Table 1 Primer sequences used for quantitative real-time PCR (qPCR)

From: Caffeine reduces hepatic lipid accumulation through regulation of lipogenesis and ER stress in zebrafish larvae

Gene

Accession

Forward primer

Reverse primer

PPAR-γ

DQ839547

5′-GGTTTCATTACGGCGTTCAC-3′

5′-TGGTTCACGTCACTGGAGAA-3′

SREBP1

NM_001105129

5′-CATCCACATGGCTCTGAGTG-3′

5′-CTCATCCACAAAGAAGCGGT-3′

PPAR-α

NM_001102567

5′-CGTCGTCAGGTGTTTACGGT-3′

5′-AGGCACTTCTGGAATCGACA-3′

ACC1

XM_009298360

5-′GCATAGGGCAGGTTTTACCA-3′

5′-GCCATCATACGAGAGCAACA-3′

FASN

XM_009306807

5-′GAGAAAGCTTGCCAAACAGG-3′

5′-GAGGGTCTTGCAGGAGACAG-3′

CD36

NM_001002363

5′-AGGCCACTGTGAACCTGAAG-3′

5′-AAGTTGGGGTTCATTCCGAC-3′

UCP2

NM_131176

5′-GAGCTTTGCTTCTGTACGCA-3′

5′-ACGAAACCCCTCTTCCTTTG-3′

CPT I

NM_001044854

5′-ACTCTCGATGGACCCTGTGA-3′

5′-CTGGATGAAGGCATCTGGAC-3′

ACO

NM_213147

5′-AAGGACATCGAGCGAATGAT-3′

5′-CTATGAAAGAGTGGAGGCCG-3′

ATF6

NM_001110519

5′-CTGTGGTGAAACCTCCACCT-3′

5′-CATGGTGACCACAGGAGATG-3′

IRE1

XM_009306258

5′-TGACGTGGTGGAAGTTGGTA-3′

5′-ACGGATCACATTGGGATGTT-3′

PERK

XM_005156585

5′-TGGGCTCTGAAGAGTTCGAT-3′

5′-TGTGAGCCTTCTCCGTCTTT-3′

BIP

NM_213058

5′-AAGAGGCCGAAGAGAAGGAC -3′

5′-AGCAGCAGAGCCTCGAAATA -3′

CHOP

XM_005166171

5′-AAGGAAAGTGCAGGAGCTGA -3′

5′-TCACGCTCTCCACAAGAAGA- 3′

Beclin-1

NM_200872

5′-AGAGCATTGAGACAAAGCGTGAA -3′

5′-TCTGCCAAGGCGGAAGTTATT-3′

ATG12

BC154046

5′-TTCATCTCACGCTTCCTCAA-3′

5′-CGTCACTTCCGAAACACTCA-3′

ATG3

NM_200022

5-′GGCTGTTTGGATATGATGAG-3′

5′-AGCAGGTGGAGGGAGATTAG- 3′

IL-1β

AY340959

5′-TGGCGAACGTCATCCAAG-3′

5′-GGAGCACTGGGCGACGCATA-3′

TNF-α

AY427649

5′-GCTTATGAGCCATGCAGTGA-3′

5′-TGCCCAGTCTGTCTCCTTCT-3′

NF-κB

BC162402

5′-GAAGCATTCAGGCTCGGTGA-3′

5′-CAGGTCTGTCGGTCCCTTTC-3′

EFL1-alpha

AY422992

5′-TACTTCTCAGGCTGACTGTG-3

5′-ATCTTCTTGATGTATGCGCT-3′