Skip to main content

Table 1 Primer list of RT-PCR and quantitative RT-PCR

From: Transgenic expression of omega-3 PUFA synthesis genes improves zebrafish survival during Vibrio vulnificus infection

Primer

Gene name

Sequence

Universal probe NO.

EF-1α

Elongation factor-1alpha

F: cctctttctgttacctggcaaa

# 73

R: cttttcctttcccatgattga

TNF-α

Tumor necrosis factor-alpha

F: aggcaatttcacttccaagg

# 158

R: aggtctttgattcagagttgtatcc

COX-2a

Cyclooxygennase-2a

F: agccctactcatcctttgagg

# 161

R: tcaaccttgtctacgtgaccata

IL-1β

Interleukin-1beta

F: acgtctccacatctcgtactca

# 12

R: tcgaaggtgtttatggagctg

IL-15

Interleukin-15

F: aggctcaggagaagactcacc

# 9

R: ggatgtcgtgctgagcaat

Lysozyme

Lysozyme

F: gggattctccattggcaac

# 48

R: ctcggtgggtcttaaacctg

NF-κB 1

Nuclear factor kappa B1

F: gcatctgcatctccgaca

# 109

R: tgcccaaattagggaaactg

Fadsd2

Fatty acid desaturase 2

F: tcaggaccggcagaaaaa

#41

R: aacagcggctgcgtttta

Elovl2

Elongase 2

F: tggacagcctatttggagaaa

#66

R: gcaaaaatgttggtgtgtagga

Elovl5

Elongase 5

F: ccaaatctcttacatggtcacg

#15

R: tcccgaacgtttcttataggtc

RT-Fadsd6 (V5-tag)

Fatty acid desaturase delta6

F: atggggggcggaggccagcag

-

R: gaatcgagaccgaggagaggg

RT-Elvol5a (V5-tag)

Elongase

F: atggagacttttaattataaac

-

R: gaatcgagaccgaggagaggg

RT- β-actin

Beta-actin

F: cacggcatcattaccaactg

-

R: gtacggccagaagcgtagag