Fig. 5From: Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular developmentNr2f1b is required for the growth of intersegmental vessels. The number of cells forming each ISV were counted in wild type control Tg(fli1a:negfp) y7 (a) and nr2f1b morphant embryos (b) at 30 hpf. c Proportional distribution of ISVs containing 1-7 cells in both conditions. d-j Time-lapse imaging of wild type Tg(kdrl:eGFP) la116 (d-f) and nr2f1b morphant (g-i) embryos to examine the extension of ISV tip cell filopodia. Confocal images at 23 hpf (red; d, g) and 26hpf (green; e, h) were merged (f, i). The extension of tip cell was quantitated by pixel intensity and shows reduced extension of tip cell in nr2f1bMO (j). k-p Time-lapse imaging of wild type Tg(fli1a:nEGFP) y7 (k-m) and nr2f1b morphant (n-p). Scale bars represent 50 μm.Back to article page