Fig 5
Examples of phospholipid metabolism reactions involving HRASLS enzymes. A, O-acyltransferase (acyl-CoA independent transacylase) reaction demonstrating the conversion of lyso PC to PC, using the sn-1 acyl chain of PC as the acyl donor. B, N-acyltransferase reaction demonstrating the acylation of the amino group of phosphatidylethanolamine using the sn-1 fatty acyl chain donated by PC, resulting in the generation of an N-acylphosphatidylethanolamine (NAPE) and a lyso PC. C, Conversion of NAPE to N-acylethanolamine (NAE) by NAPE-phospholipase D-mediated hydrolysis. D, Phospholipase A1 (PLA1) reaction demonstrating the cleavage of a fatty acyl chain from the sn-1 position of PC, resulting in the liberation of a free fatty acid and a 1-hydroxy-2-acyl-lyso PC. E, Phospholipase A2 (PLA2)-mediated conversion of PC to lyso PC by hydrolysis of a fatty acyl chain at the sn-2 position