Fig. 8
From: YC-1 induces lipid droplet formation in RAW 264.7 macrophages
PKG inhibitor reduces YC-1-stimulated lipid accumulation in macrophages and ox-LDL-mediated foam cells. a PKG inhibitor reduced YC-1-induced lipid accumulation in macrophages. RAW 264.7 macrophages were treated with or without 3 μM KT5823, and then exposed to 20 μM YC-1 for 24 h. After fluorescence staining with AdipoRed™ and Hoechst, the mean fluorescence intensity (AdipoRed) per cell was detected and analyzed by an HCS reader (n = 5). b PKG inhibitor reduced YC-1-induced lipid accumulation in ox-LDL-mediated foam cells. RAW 264.7 macrophages were treated with or without 3 μM KT5823, and then exposed to 20 μM YC-1 and 50 μg/mL ox-LDL for 24 h. After fluorescence staining with AdipoRedTM and Hoechst, the mean fluorescence intensity (AdipoRed) per cell was detected and analyzed by an HCS reader (n = 5). * indicates P < 0.05 compared to the control group; § indicates P < 0.05 compared to the YC-1-treated group; # indicates P < 0.05 compared to the ox-LDL-treated group; indicates P < 0.05 compared to the YC-1 and ox-LDL-treated group