Skip to main content
Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Blocking heme oxygenase-1 by zinc protoporphyrin reduces tumor hypoxia-mediated VEGF release and inhibits tumor angiogenesis as a potential therapeutic agent against colorectal cancer

Fig. 3

ZnPP inhibited cell proliferation and decreased HIF-1α levels in HCT-15 cells. a HCT-15 cells cultured in normoxia were treated with hemin, a HO-1 inducer, or ZnPP, a HO-1 inhibitor, using a dose-dependent manner, and the cell number was counted and compared. The cells without treatment were used as control (Ctrl). ZnPP (10 μM) significantly inhibited the HCT-15 cell viability, but hemin did not. b & c The treated cells were harvested and investigated (n = 3) for detecting the expressions of HO-1, HIF-1α, ER stress marker GRP78, and β-actin. Hemin increased HIF-1α and HO-1 (without significance), but ZnPP decreased HIF-1α and HO-1. The ratio of GRP78/β-actin was equivalent among the groups as an indicator excluding the treated effects derived from hemin or ZnPP. d Furthermore, apoptosis was not detected in HCT-15 cells treated with 10 μM of ZnPP, whereas 10 μM of cisplatin was used as a positive control to induce apoptosis, indicating that ZnPP inhibited HCT-15 cell proliferation without leading to apoptosis. *p < 0.05. ***p < 0.001. # p < 0.05 compared to ctrl

Back to article page