Fig. 3

SB203580 induces G-CSF expression in RAW264.7 cells in the absence of LPS stimulation. a RAW264.7 cells were left untreated (lane 1) or were incubated with LPS for 15 to 60 min (lanes 2–4), then phosphorylated p38, total p38, and β-actin levels were analyzed by Western blotting. b RAW264.7 cells were incubated with DMSO or 10 μM SB203580 for 0 to 8 h, then levels of G-CSF and GAPDH (internal control) mRNA were determined by RT-PCR and analyzed by gel electrophoresis (upper panels). The G-CSF mRNA levels were normalized to those for GAPDH mRNA and expressed relative to the value in the DMSO-treated cells at 0 h (relative value = 1) (lower panels). The results are the mean ± SD for three independent experiments. *p < 0.05, compared to the DMSO-treated cells