Fig. 7

Mutation of the SLDE abolishes the SB203580-induced increase in luciferase activity in cells transfected with Gp-LUC-3’UTR. a The sequence and structure of the wild-type SLDE (WT) in the 3’UTR of G-CSF and the three SLDE mutants (M1, M2, and M3). b RAW264.7 cells were co-transfected with one of the reporter plasmids (Gp-LUC, Gp-LUC-3’UTR, or Gp-LUC-3’UTR mutants one to three) and control plasmid (phRLTK), then, 4 h later, were either treated with DMSO (D) or 10 μM SB203580 (SB) for 20 h (left 2 columns) or were pretreated for 30 min with DMSO or 10 μM SB203580, then LPS was added and the cells incubated for a further 20 h (right 2 columns), then the Photinus and Renilla luciferase activities were measured. The Photinus luciferase activity was normalized to the Renilla luciferase activity and expressed relative to the value for the DMSO-treated control for the same reporter, set as 1. The values are the mean ± SD for three independent experiments. *p < 0.05, compared to the corresponding DMSO-treated control. n.s., not significant