SB203580 increases G-CSF production via a stem-loop destabilizing element in the 3’ untranslated region in macrophages independently of its effect on p38 MAPK activity

Table 1 Equences of the primers used in this study

Primers for RT-PCR
Primers	Sequence
mouse G-CSF	Forward 5’-CTCAACTTTCTGCCCAGAGG-3’
	Reverse 5’-CTGGAAGGCAGAAGTGAAGG-3’
human G-CSF	Forward 5’-CACTCTGGACAGTGCAGGAAG-3’
	Reverse 5’-CGACACCTCCAGGAAGCTCTG-3’
mouse IL-1β	Forward 5’-GACCTTCCAGGATGAGGACA-3’
	Reverse 5’-AGGCCACAGGTATTTTGTCG-3’
human & mouse GAPDH	Forward 5’-AAAGGATCCACTGGCGTCTTCACCACC-3’
	Reverse 5’-GAATTCGTCATGGATGACCTTGGCCAG-3’
Primers for RT-qPCR
Primers	Sequence
mouse G-CSF	Forward 5’-TTGGCAACATCCAGCTGAAG-3’
	Reverse 5’-GCAGGCTCTATCGGGTATTTCC-3’
human G-CSF	Forward 5’-TCCCCATCCCATGTATTTATCT-3’
	Reverse 5’-AACTCAGAAATGCAGGGAAGGA-3’
human & mouse GAPDH	Forward 5’-GGCATTGTGGAAGGGCTCAT-3’
	Reverse 5’-GACACATTGGGGGTAGGAACAC-3’
Photinus luciferase	Forward 5’-GCCTGAAGTCTCTGATTAAGT-3’
	Reverse 5’-ACACCTGCGTCGAAGA-3’
Renilla luciferase	Forward 5’-GCAAGGGTTGGTCGTGAGG-3’
	Reverse 5’-TCATCCGTTTCCGTTCTG-3’
Primers for plasmid construction
Primers	Sequence
mouse GAPDH promoter	Forward 5’-TTACGCGTGATGATGGAGGACGTGATG-3’
	Reverse 5’-ATCAGATCTGCAGGAGAAGAAAATGAG-3’
mouse G-CSF 3’UTR	Forward 5’-AGTCTAGACCTGAGCAGAAAGCCCTTTCC-3’
	Reverse 5’-AGTCTAGAGGAACACCACACTTTATTATCCGCA-3’

The underlined sequences are restriction enzyme sites created to facilitate cloning

ISSN: 1423-0127