Fig. 2
From: Advancement and applications of peptide phage display technology in biomedical science
Biopanning selection of phage-displayed peptide libraries using serum samples. The phage-displayed peptide library was precleared using normal human sera and selected with serum antibodies (Abs) from patients with candidate diseases. After three rounds of biopanning, immunopositive phage clones were screened by ELISA. Disease-specific epitopes were further identified and characterized by synthetic peptide binding and competitive-inhibition assays. Phage-displayed disease-specific epitopes can be used to determine microbiological origins, study immunotyping, or provide information for the development of diagnostic reagents and vaccines