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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Ethanol induces cytostasis of cortical basal progenitors

Fig. 2

Ethanol inhibits the proliferation of basal progenitors. a Cells were treated with or without ETOH [4 mg/ml (86 mM)] for 8, 12 and 24 h and subsequently cells were counted by Vi-Cell cell viability analyzer. Statistical analysis was determined by one-way analysis of variance (ANOVA) and Newman-Keul’s posthoc test. *p < 0.05 when compared with untreated control. b A measure of proliferation index in the last cycle after 14 h treatment of ethanol in a chronic intermittent ethanol exposure (CIE) model as mentioned in materials and methods. Significance was determined by one-way analysis of variance (ANOVA) and Newman-Keul’s posthoc test. *p < 0.05 when compared with untreated control. c To further confirm the anti-proliferative effect of ETOH, classical BrdU based cell cycle progression analysis was performed. Cells were pulse-labeled with BrdU during the last 4 h of treatment before harvesting at 24 h. (Top): Representative scatter plots with the log FITC anti-BrdU staining versus PI staining. (Bottom): The graph illustrates the percentage of cells in synthesis phase. Sub G0-G1 phase depicts apoptotic cells. *p < 0.05 when compared with control as assessed by Student’s t-test

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