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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Ethanol induces cytostasis of cortical basal progenitors

Fig. 5

In utero ethanol exposure decreases Tbr2 and cyclin D1 positive cell numbers in fetal cerebral cortex. Pregnant Sprague-Dawley rats were administered ETOH 3.5 g/kg body weight or iso-caloric dextrose (CONT) by gastric intubation by gavage at 12 h interval for 3 days starting embryonic day 15 (E15) and sacrificed at embryonic day 18 (E18). Embryos were removed and the cerebral cortex was dissected from the rest of the brain. Tissues were isolated into single cells by mechanical disruption and were dual-labeled with Tbr2 antibody conjugated with PE and cyclin D1 antibody conjugated with FITC. a Tbr2-positive cells were identified by gating on the single parameter PE histogram. b Tbr2-positive cells were further analyzed for the expression of cyclin D1 by gating on single parameter FITC histogram. (a & c, analyzed by FlowJo software). c The bars represent percent Tbr2-positive cells in controls and ethanol treatment (d) Percent positive cyclin D1 cells within Tbr2-positive cells. (b & d analyzed by FACSDiva software). Data was analyzed by Student’s t-test. *p < 0.05 when compared with control. n = 4

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