Fig. 4

Transduction efficiency of denatured Tat-SOD into differentiated PC12 cells. a Differentiated PC12 cells plated in a six-well plate were treated with 1.5 μM of denatured TatSOD in the culture medium for 2 h. b Differentiated PC12 cells were incubated for various time intervals with 1.5 μM of denatured Tat-SOD. Then, the transduced fusion proteins in the cells were analyzed by immunofluorescence microscopy. DAPI, nuclear staining; Cy3, SOD1 staining. c PC12 cells were incubated with quantum dots (QDot 560 nm)-conjugated Tat-SOD molecules for 2 or 4 h with different doses of the conjugate (as indicated). Cells were later stained with DAPI. Results were observed using fluorescence microscopy with an FITC filter