Fig. 1

Schematic diagram of gene construction and identification of fusion proteins. a The genes encoding CNGRC-yCD and yCD were cloned into the pET28a expression vector using a PCR cloning strategy, and digested with the restriction enzymes, Nco I and Xho I. b The respective purified yCD and CNGRC-yCD fusion proteins were identified by Coomassie brilliant blue stained gels (left panel) and Western blot using His₆-tag-specific antibody (right panel). The experiments were completed at least three times with similar results