The prognostic effect of PTEN expression status in colorectal cancer development and evaluation of factors affecting it: miR-21 and promoter methylation

Table 1 The used primers in this study

Amplified factor	primers 5′ to 3′		Product size (bp)	AT (°C)	Genbank accession number
miR-21	S	TGTCGGGTAGCTTATCAGAC	~90	55	NR_029493
	AS	Adapter primer in kit
U6-snRNA	S	GCTTCGGCAGCACATATAC	~120	55	NR_004394
	AS	Adapter primer in kit
PTEN	S	ACCAGAGACAAAAAGGGAGTA	173	56	NM_000314
	AS	ACCACAAACTGAGGATTGCA
HPRT	S	TGGACTAATTATGGACAGGACT	219	56	NM_000194
	AS	CCTGTTGACTGGTCATTACAAT
PTEN Promoter
Unmethylated (−300)^a	S-U	TGGGTTTTGGAGGTTGTTGGT	173	55	NG_007466.2
Unmethylated (−300)^a	AS-U	ACTTAACTCTAAACCACAACCA
Methylated (−298)^a	S-M	GGTTTCGGAGGTCGTCGGC	155	57
Methylated (−298)^a	AS-M	CAACCGAATAATAACTACTACGACG

S sense, AS antisense, U unmethylated, M methylated, AT annealing temperature
^aDistance of the 5′ nucleotide of the sense primer from the transcription start site of PTEN gene

ISSN: 1423-0127