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Fig. 9 | Journal of Biomedical Science

Fig. 9

From: Betulinic acid enhances TGF-β signaling by altering TGF-β receptors partitioning between lipid-raft/caveolae and non-caveolae membrane microdomains in mink lung epithelial cells

Fig. 9

The immunofluorescence localization of TβR-II-HA and caveolin-1 in Mv1Lu cells treated with and without BetA. The Mv1Lu cells transiently expressing TβR-II-HA were treated with or without 5 μg/mL of BetA at 37 °C for 1 h. The cells were then fixed with cold methanol and incubated with a mouse antibody against HA (panels a and b) and a rabbit antibody against caveolin-1 (panels b and e), followed by incubation with Rhodamine-conjugated donkey anti-mouse antibody or FITC-conjugated goat anti-rabbit antibody. Fluorescence in cells was examined using a confocal microscope: Bar, 20 μm. The white arrows indicate the colocalization of TβR-II-HA and caveolin-1 at the cell surface and endocytic vesicle (panel c, enlarged picture in lower right corner.) before BetA treatment. (panel f, enlarged picture in upper right corner.) BetA treatment reduces the colocalization of TβR-II-HA and caveolin-1

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Journal of Biomedical Science

ISSN: 1423-0127