Fig. 7

Macrophages concentrate near the peritoneal surface in wild type and Sdc1 ^-/- mice with subacute peritoneal dialysis. Sections of the abdominal wall from S. aureus-infected animals that were treated with peritoneal dialysis for 4 weeks were prepared for immunohistochemistry and incubated with (a) anti-F4/80 antibody to identify macrophages or (b) the isotype control antibody and counterstained with Gill’s hematoxylin No. 2. In some areas of the sections, F4/80⁺ cells were found in the fibrotic peritoneum concentrated near the surface (brown color). Representative images, n = 4 mice, scale bar = 50 μm