Fig. 9
Syndecan-1 does not modulate leukocyte recruitment to the parietal peritoneum microcirculation in the early response to S. aureus infection. Wild type and Sdc1 -/- mice were injected intraperitoneally with 1.8 × 108 colony-forming units of S. aureus. After 4 h, the microcirculation of the parietal peritoneum was imaged with intravital microscopy. The numbers of (a) rolling and (b) adherent leukocytes in the peritoneal venules, as well as the number of (c) extravascular leukocytes, were quantified. S. aureus infection significantly increased the number of adherent and extravascular leukocytes compared with saline controls. However, there were no significant differences in leukocyte recruitment between the wild type and Sdc1 -/- mice. Data recorded as mean ± SEM and analyzed with ANOVA with Bonferroni correction, 4–6 venules averaged per count, n = 4 mice, **p < 0.001 and ***p < 0.0001 compared with saline