Syndecan-1 (CD138) deficiency increases Staphylococcus aureus infection but has no effect on pathology in a mouse model of peritoneal dialysis

Table 1 Peritoneal lavage leukocyte counts and differential blood leukocyte counts

Treatment/strain	Total peritoneal cells × 10⁶	Total blood cells × 10⁹ L^-1	Blood neutrophils	Blood lymphocytes	Blood monocytes
Baseline wild type	3.52 ± 0.43	4.08 ± 0.67	0.99 ± 0.27	3.06 ± 0.42	0.04 ± 0.01**
Baseline Sdc1 ^-/-	2.78 ± 0.28	4.56 ± 0.68	1.14 ± 0.26	3.40 ± 0.51	0.02 ± 0.01
Non-PD + S. aureus wild type	25.78 ± 3.43***	4.98 ± 0.65	1.80 ± 0.20	3.14 ± 0.53	0.34 ± 0.07
Non-PD + S. aureus Sdc1 ^-/-	15.94 ± 1.57***	3.62 ± 0.49	1.58 ± 0.31	2.01 ± 0.25	0.03 ± 0.02**
PD + S. aureus wild type	31.65 ± 1.90***	3.12 ± 0.33	0.79 ± 0.12*	2.14 ± 0.27	0.19 ± 0.04
PD + S. aureus Sdc1 ^-/-	22.42 ± 1.89***	3.29 ± 0.52	1.05 ± 0.16	2.11 ± 0.41	0.13 ± 0.03

At the end of the peritoneal dialysis (PD) period, mice were infected intraperitoneally with 1.8 × 10⁸ colony-forming units of S. aureus and 4 h after, peritoneal lavage was performed and blood was collected via cardiac puncture for enumeration of leukocytes. Data recorded as mean ± SEM and analyzed with ANOVA with Bonferroni correction, 6 counts averaged per mouse, n = 8–14 mice, *p < 0.01 and **p < 0.001 compared with non-PD wild type infected mice, ***p < 0.0001 compared with baseline values. S. aureus-infected mice had significantly increased numbers of peritoneal leukocytes compared with baseline but systemic leukocyte counts did not differ significantly

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