Fig. 2

Vimentin involved in the SARS-CoV spike-ACE2 complex on plasma membrane at different time points. Vero E6 cells were incubated with SARS-CoV VLPs for 10, 30 and 60 min at 37 °C. Cell lysates were then prepared and subjected to immunoprecipitation (IP) with anti-ACE2 antibodies, followed by Western blot (WB) analysis with anti-vimentin antibodies (Sigma). Cell lysates without the process of immunoprecipitation (10 % input) were analyzed in parallel with anti-vimentin and anti-ACE2 antibodies as the protein loading controls