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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Surface vimentin is critical for the cell entry of SARS-CoV

Fig. 5

Direct interaction between vimentin and SARS-CoV spike protein analyzed by far-Western blot analysis. a Far-Western blot analysis. Protein lysates prepared from Vero E6 cells were blotted on PVDF membrane and subjected to far-Western blot analysis with anti-V5 epitope antibodies (left panel) following a pre-incubation of the membrane with the purified V5-tagged recombinant SARS-CoV spike protein. The right panel showed the Vero E6 cells pre-incubated with anti-vimentin antibodies (Sigma) prior to the treatment of SARS-CoV spike protein. b Western blot analysis. The same set of protein lysates blotted on PVDF membrane was subjected to Western blot analysis with anti-vimentin antibodies to serve as a positive control. c Coomassie blue staining. Coomassie blue staining showed the total proteins on the gel. BSA loaded in parallel was used as a negative control

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